Activity 2: Grassland ecosystem service indicator development
Activity 2.1 Intensive native grassland sampling (Bennett/Prager/Cattani/Gibbs)
To develop the ecosystem service indicators and identify their relationship with plant diversity, we will intensively study at least 12 native grasslands, although this number may increase if sites containing all focal species cannot be found. To select appropriate sites for our study, we will work with our partner organizations to identify sites managed by these groups or their members that contain the species of interest. In addition to working with our partners, we will use publicly available resources to identify observations of the focal species (e.g., iNaturalist, Global Biodiversity Information Facility). Observations of the five focal species selected in Act1.1 will be integrated to identify potential sampling sites where the range of all focal species overlap. Potential sites will be surveyed by one of three teams of students (one graduate plus one undergraduate student) to determine whether the populations of the focal species are sufficiently large for sampling (>20 individuals). As the sites are likely to cover significant geographic area and the flowering windows are time sensitive, student teams will be assigned one of three regions (southern Manitoba, southern Saskatchewan & Alberta, and central Saskatchewan & Alberta), although the precise regions will depend on the location of the potential sites. Sites with less than 15 mature individuals per species for at least three focal species will be excluded from sampling.
Milestone 2.1 (Q4 Y1): Intensive native grassland sampling completed.
Milestone 2.2 (Q3 Y2): Non-genomic plant sampling complete.
Milestone 2.3 (Q4 Y2): Act2 soil and eDNA genomics complete.
Milestone 2.4 (Q1 Y4): Plant genomics and related analyses complete.
Milestone 2.5 (Q4 Y3): Bee phylogenomic analyses complete.
The purpose of the intensively sampled grasslands is to examine among- and within-site variability in the potential for carbon sequestration and the provisioning of other ecosystem services, its relationship to plant species composition and diversity, and intraspecific variation in the potential for providing ecosystem services. These data will allow us to develop genomic indicators of ecosystem service potential (Del3), and their analysis will allow us to quantify how species and genetic diversity affect GHG-associated ecosystem services within and among native grasslands (Del2). Bennett, Prager, Gibbs, and Cattani will coordinate sampling of field sites (Act2.1), with actual field sampling conducted by PhD1 (Gibbs), MSc3 (Prager), and four undergraduate students. Bennett and Prager will coordinate sampling in Saskatchewan and Alberta, whereas Cattani and Gibbs will coordinate sampling in Manitoba. To standardize sampling, all students will be centrally trained by the co-Investigators, who have considerable expertise in plant, insect, and soil sampling. Technician 1, supervised by Bennett and Prager, will oversee all molecular analyses and train students in the relevant techniques. Bennett and Ko, with expertise in ecoinformatics and machine learning, will supervise data analysis to be conducted by PDFs 1&2 and PhD2 (Ko).